Dr. Li will present data demonstrating the feasibility and reliability of a standardized brief quantitative sensory testing (QST) protocol used in youth with various acute pain conditions, including post-musculoskeletal (MSK) surgery and injury-related pain. Dr. Li will present the methodology of this protocol adapted for use in youth with musculoskeletal pain, considering developmental appropriateness, relevance to MSK pain, participant burden, and application across multiple geographical study sites. Dr. Li will discuss technical aspects of the protocol, including the modality, intensity, and duration of stimuli used, as well as the locations on the body where the stimuli are applied. The presentation will also include data on the influence of demographic (age, sex, ethnicity) and personal contextual factors (momentary pain, menstrual cycle, analgesic use) on key QST parameters (e.g., threshold, tolerance, temporal summation of pain, conditioned pain modulation) in youth with acute MSK pain.